Description

Whole Exome Sequencing (WES) Focus your genomic research on protein-coding regions with Dextrose Technologies Pvt. Ltd. Our whole exome sequencing service combines sequencing library preparation, targeted exome enrichment, and paired-end sequencing to generate data for variant discovery and genetic research. The service catalogue specifies the Twist Human Core Exome Kit, making this workflow intended for human exome sequencing. Projects involving other organisms require a separately confirmed capture strategy. Service Overview Submitted genomic DNA is used to prepare a sequencing library. Exome enrichment captures the regions targeted by the Twist Human Core Exome Kit, followed by 2 × 150 bp paired-end sequencing. The catalogue specifies 6 Gb of sequencing data per sample. This provides a foundation for downstream reference alignment, detection of single nucleotide variants and small insertions or deletions, and variant annotation. Applications • Research into variation within targeted protein-coding regions. • Identification of candidate variants for further investigation. • Comparative analysis of research samples. • Studies linking genetic variation with research phenotypes. • Generation of data for downstream functional interpretation. • Support for academic, biotechnology, and genomics research projects. Sample Requirements • Sample type: Extracted human genomic DNA. • Required DNA quantity: 3 µg per sample. The catalogue does not specify DNA concentration, submission volume, or purity thresholds. Confirm acceptance criteria and transport conditions with our team before dispatch. Submission Information Clearly label each sample and provide sample identifiers, sample source, DNA extraction method, total DNA quantity, and available quality assessment results. Share the study objective, sample relationships, intended comparisons, and reference genome preference where applicable. Specialised requirements, including tumour–normal comparisons or low-frequency variant detection, should be discussed before confirming the workflow. Sequencing Deliverables • Sequencing library preparation. • Exome enrichment using the Twist Human Core Exome Kit. • 2 × 150 bp paired-end sequencing. • 6 Gb of sequencing data per sample. The stated data yield does not guarantee a particular mean depth or uniform coverage across every target region. Confirm coverage expectations, sequence file formats, and data transfer arrangements during project planning. Additional Bioinformatics Analysis The catalogue lists WES bioinformatics as a separate service. When included in the agreed scope, it covers: • Alignment of quality-filtered sequencing reads to a reference genome. • Variant calling for single nucleotide variants and small insertions or deletions. • Variant annotation. • Delivery of annotated variant results. Confirm the reference build, annotation scope, filtering approach, and output formats before analysis begins. Interpretation WES focuses on regions included in the capture design. Coverage may vary between targets, and the workflow does not assess every type of genetic variation equally. Variant detection and annotation support research interpretation. A detected variant does not by itself establish its biological effect. Clinical interpretation, diagnostic reporting, and confirmatory testing require separately agreed services where applicable. Turnaround Time Sequencing: 3–4 weeks. Bioinformatics analysis: Enquire for the applicable timeline. Service Coverage Based in Bengaluru, we support projects across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai.