Description

Whole Genome Sequencing — Fungal Explore the genetic content of fungal isolates with Dextrose Technologies Pvt. Ltd. Our fungal whole genome sequencing service generates DNA sequence data for genomic characterisation, comparative research, and downstream bioinformatics analysis. The service supports research involving yeasts and filamentous fungi. Sequencing across the genome provides a foundation for investigating genes, sequence variation, and genetic features relevant to your organism and study objectives. Service Overview Submitted fungal genomic DNA is used to prepare a sequencing library, followed by 2 × 150 bp paired-end sequencing using the Illumina HiSeq platform specified in our service catalogue. The resulting reads can support reference-based analysis or de novo genome assembly, depending on the agreed project scope. Sequencing depth and data output should be selected according to genome size, complexity, and research objectives. Applications • Genomic characterisation of fungal isolates. • Comparative studies between fungal strains. • Investigation of genetic variation. • Characterisation of industrial and fermentation-associated fungi. • Research involving agricultural and environmental fungal isolates. • Generation of data for downstream gene annotation and functional studies. • Support for academic, dissertation, PhD, and biotechnology projects. Sample Requirements • Sample type: Extracted fungal genomic DNA. • Required DNA quantity: 3 µg per sample. The catalogue does not specify DNA concentration, submission volume, purity thresholds, or sequencing coverage. Confirm these acceptance criteria and the required data output with our team before dispatch. Submission Information Clearly label each sample and provide the isolate identifier, organism name where known, sample source, DNA extraction method, and available quality assessment results. DNA should represent the intended fungal isolate. Mixed cultures or contamination may complicate genome analysis. Discuss samples with uncertain purity, low DNA quantity, or degraded DNA before submission. Share the expected genome size, ploidy information, and reference genome details where available. For comparative studies, describe the relationship between isolates and the comparisons required. Sequencing Deliverables • Genomic DNA sequencing library preparation. • 2 × 150 bp paired-end Illumina HiSeq sequencing. Confirm the read count, coverage target, sequence file formats, and data transfer arrangements during project planning, as these are not specified in the catalogue entry. Additional Bioinformatics Analysis The catalogue lists WGS bioinformatics separately. Where suitable for the fungal dataset, the reference-based package includes: • Alignment of quality-filtered reads to a reference genome. • Variant calling. • Variant quality assessment. • Annotation of identified variants. • Annotated variant results in spreadsheet format. De novo assembly, fungal gene prediction, functional annotation, phylogenetic comparison, and genome visualisation should be discussed and explicitly included in the agreed scope where required. Interpretation Genome recovery depends on DNA quality, sequencing depth, contamination, genome complexity, and heterozygosity. Short-read sequencing may produce a fragmented draft assembly; a complete chromosome-level genome is not guaranteed. Gene annotations and functional predictions require interpretation alongside reference information and experimental evidence. Turnaround Time Sequencing: 3–4 weeks. Bioinformatics analysis: Enquire for the applicable timeline. Service Coverage Based in Bengaluru, we support projects across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai.