Description

Discover the microbial composition and functional potential of your samples with Dextrose Technologies Pvt. Ltd., Bengaluru. Our shotgun metagenomic data analysis service helps researchers and industry teams convert complex sequencing datasets into meaningful biological insights. We support gut microbiome, soil, water, food, agricultural, and industrial microbiome research. Analysis is tailored to your study objectives, sequencing depth, sample metadata, and experimental design. Analysis Scope • Raw sequencing data quality assessment, adapter trimming, and quality filtering. • Host-read removal where an appropriate reference is available. • Taxonomic profiling of bacteria, archaea, fungi, and DNA viruses, subject to sequencing coverage and database representation. • Relative abundance estimation and microbial community comparison. • Alpha and beta diversity analysis. • Functional gene annotation and metabolic pathway profiling. • Statistical comparison between study groups where adequate replication and metadata are available. • Screening for antimicrobial resistance and virulence-associated genes, when included in the project scope. • Optional metagenome assembly, gene prediction, and metagenome-assembled genome reconstruction, subject to data suitability. Deliverables • A comprehensive report covering methodology, results, biological interpretation, and limitations. • Quality-control summaries and read-retention statistics. • Taxonomic abundance tables at relevant classification levels. • Diversity results and community comparison outputs. • Functional annotation and pathway abundance tables. • Relevant high-resolution figures, such as stacked abundance plots, heatmaps, diversity plots, and ordination plots. • Statistical result tables for agreed comparisons. • Processed analysis outputs and relevant software, database, and parameter details. • Assembly, genome-bin, antimicrobial resistance, or virulence-screening outputs when included in the agreed scope. • A results discussion to clarify findings and guide further research. Applications Microbiome research, environmental monitoring, agricultural studies, food microbiology, microbial ecology, and industrial biotechnology. Required Inputs Raw shotgun sequencing files in FASTQ/FASTQ.gz format, sample identifiers, study objectives, group information, and relevant sample metadata. Final deliverables, pricing, and turnaround time depend on dataset size, sequencing quality, study design, and selected analysis modules. Functional results describe genetic potential; they do not directly measure gene expression or microbial activity.