Description

Amplify your target DNA and confirm its sequence with Dextrose Technologies Pvt. Ltd. Our PCR & Sequencing service combines polymerase chain reaction (PCR) amplification with Sanger sequencing to generate nucleotide sequence information for identification and confirmation of selected DNA targets. This service supports academic research, molecular biology studies, biotechnology projects, and industrial R&D requiring targeted DNA sequence verification. Service Overview PCR amplifies a selected DNA region using suitable primers. The amplified product is then processed for Sanger sequencing, generating a DNA sequence and its corresponding chromatogram. The sequence output can support subsequent comparison with an expected sequence or reference database. Any additional alignment, identification, or interpretation requirements should be discussed when submitting the project. Applications • Amplification and sequencing of selected DNA regions. • Confirmation of target sequences in molecular biology experiments. • Verification of amplified gene fragments. • Generation of sequence data for downstream identification and comparative analysis. • Examination of nucleotide differences within the sequenced region. • Support for dissertation, PhD, and biotechnology research projects. Sample Requirements As listed in our service catalogue: • DNA sample volume: 10–20 µL per sample. • DNA concentration: 50–100 ng/µL. • Primer concentration: 10 pmol/µL. • Primer volume: 10 µL. Provide the forward and reverse primer sequences and clearly identify the primers supplied for amplification and sequencing. Confirm primer quantities and the required sequencing direction with our team before dispatch. Submission Information Label each sample and primer tube clearly. Include sample identifiers, sample type, target gene or region, expected amplicon size, and the purpose of the analysis. Where available, share an expected reference sequence and any previous PCR results. Samples with uncertain DNA quality, low concentration, or known amplification difficulties should be discussed before submission. Deliverables • AB1 chromatogram files containing sequencing traces and base calls. • FASTA files containing the generated nucleotide sequences. • Sequencing output in PDF format. Sequence quality and usable read length depend on template quality, amplification specificity, primer performance, and sequence characteristics. Mixed amplification products may affect the clarity of sequencing results. Turnaround Time 3–4 weeks, as listed in our service catalogue. Service Coverage Based in Bengaluru, Dextrose Technologies supports researchers and organisations across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai. Contact Us Share your target details, sample information, and project requirements for submission guidance. Dextrose Technologies Pvt. Ltd. Phone: +91 9880405558 Email: info.dextrosetech@gmail.com Website: www.dextrosetech.com