Description

Explore gene expression in eukaryotic samples with Dextrose Technologies Pvt. Ltd. Our transcriptome sequencing service generates RNA sequencing data to support research into expressed genes, cellular responses, and biological differences between experimental conditions. The service uses poly(A) enrichment followed by RNA-Seq library preparation and paired-end sequencing. This approach focuses primarily on polyadenylated transcripts, making it suitable for many messenger RNA studies in eukaryotic organisms. Service Overview Submitted total RNA undergoes poly(A) enrichment to capture polyadenylated RNA. The enriched material is used to prepare sequencing libraries, followed by 2 × 150 bp paired-end sequencing using the Illumina HiSeq platform specified in our service catalogue. The resulting sequencing data provides a foundation for downstream read alignment, transcript abundance estimation, and gene expression analysis. Applications • Gene expression profiling in eukaryotic samples. • Investigation of cellular responses to treatments or environmental conditions. • Comparative studies between experimental and control groups. • Identification of candidate genes for further validation. • Research into development, stress responses, and biological pathways. • Support for academic, biotechnology, agricultural, and industrial R&D projects. Sample Requirements • Sample type: Total RNA from a eukaryotic source. • Required quantity: 5 µg total RNA per sample. The catalogue does not specify RNA concentration, submission volume, or an RNA integrity threshold. Confirm these acceptance criteria and transport conditions with our team before dispatch. Submission Information Clearly label each sample and provide the organism name, sample source, extraction method, total RNA quantity, and available quality assessment results. For comparative projects, include treatment groups, control groups, biological replicate information, and study objectives. Share reference genome and annotation details where available. RNA quality influences library preparation and sequencing outcomes. Discuss degraded samples or unusual sample types before submission. Studies targeting non-polyadenylated transcripts may require a different enrichment strategy. Sequencing Deliverables • Poly(A) enrichment. • RNA-Seq library preparation. • 2 × 150 bp paired-end sequencing using Illumina HiSeq. • 10 million reads per sample, counted as R1 + R2. The stated output is 10 million individual reads across R1 and R2, rather than 10 million read pairs. Additional Bioinformatics Analysis The catalogue lists downstream bioinformatics as a separate service. When included in the agreed scope, it covers: • Alignment of quality-filtered reads to a reference genome. • Transcript abundance estimation. • Differential gene expression analysis. • Identification of statistically significant up- and down-regulated genes. • Gene Ontology functional annotation and pathway analysis. Statistical comparisons require suitable biological replication and an appropriate experimental design. Confirm the analysis scope and output formats when planning your project. Turnaround Time Sequencing: 3–4 weeks. Bioinformatics analysis: Enquire for the applicable timeline. Service Coverage Based in Bengaluru, Dextrose Technologies supports projects across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai. Contact Us Dextrose Technologies Pvt. Ltd. Phone: +91 9880405558 Email: info.dextrosetech@gmail.com Website: www.dextrosetech.com