Description

Small / Micro RNA Sequencing Explore small RNA expression with Dextrose Technologies Pvt. Ltd. Our small RNA sequencing service generates sequencing data for profiling short RNA molecules, including microRNAs, to support expression studies and research into gene regulation. MicroRNAs are small regulatory RNAs that influence gene expression. Sequencing helps researchers examine their abundance and generate data for comparisons across biological conditions, treatments, or sample groups. Service Overview Submitted total RNA is used to prepare a small RNA sequencing library, followed by 1 × 50 nucleotide single-end sequencing using the Illumina HiSeq platform specified in our service catalogue. The catalogue specifies an output of 10–15 million reads per sample. The resulting data provides a foundation for downstream small RNA identification, annotation, and expression analysis, subject to the agreed bioinformatics scope. Applications • MicroRNA expression profiling. • Investigation of small RNAs associated with experimental treatments. • Comparative expression studies across biological conditions. • Identification of candidate microRNAs for further validation. • Research into development, stress responses, and gene regulation. • Support for academic, biotechnology, dissertation, and PhD projects. Sample Requirements • Sample type: Total RNA. • Required quantity: 5 µg total RNA per sample. Use an extraction method that retains the small RNA fraction. Some RNA purification methods may exclude short RNA molecules, affecting suitability for this service. The catalogue does not specify concentration, submission volume, or RNA integrity thresholds. Confirm sample acceptance criteria and transport conditions with our team before dispatch. Submission Information Clearly label each sample and provide the organism name, sample source, extraction method, total RNA quantity, and available quality assessment results. Indicate whether the extraction method preserves small RNAs. For comparative projects, provide treatment groups, controls, biological replicate information, and study objectives. If you intend to submit low-input RNA, extracellular vesicle RNA, or other specialised material, discuss compatibility before submission, as these sample types are not specified in the catalogue entry. Sequencing Deliverables • Small RNA-Seq library preparation. • 1 × 50 nucleotide single-end sequencing using Illumina HiSeq. • 10–15 million sequencing reads per sample. Confirm sequence file formats, data transfer arrangements, and any additional quality-control outputs during project planning. Bioinformatics Analysis The sequencing entry does not specify a dedicated small RNA analysis package. Discuss and confirm any additional requirements, such as: • Adapter trimming and read quality filtering. • Small RNA length-distribution assessment. • Identification and quantification of known microRNAs. • Differential expression analysis. • Exploratory novel microRNA prediction. • Target prediction and functional interpretation. These analyses should be explicitly agreed before the project begins. Statistical comparisons require suitable biological replication and an appropriate experimental design. Interpretation Recovery of small RNAs depends on extraction, RNA quality, library preparation, and sequencing depth. Total read count does not equal the number of microRNA reads. Predicted regulatory relationships require further validation. Turnaround Time Sequencing: 3–4 weeks. Additional analysis: Confirm the applicable timeline. Service Coverage Based in Bengaluru, we support projects across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai.