Description

Explore the genetic content of bacterial isolates with Dextrose Technologies Pvt. Ltd. Our bacterial whole genome sequencing service generates DNA sequence data for genomic characterisation, comparative research, and downstream bioinformatics analysis. Whole genome sequencing examines DNA across the bacterial genome, providing a foundation for investigating genes, sequence variation, and features relevant to the organism and study objectives. Service Overview Submitted bacterial genomic DNA is used to prepare a sequencing library, followed by 2 × 150 bp paired-end sequencing using the Illumina HiSeq platform specified in our service catalogue. The resulting reads can support reference-based analysis or genome assembly, depending on the project scope and available reference information. Required sequencing depth and data output should be agreed before starting the project. Applications • Genomic characterisation of bacterial isolates. • Comparison of related strains. • Investigation of genetic differences between isolates. • Characterisation of candidate probiotic and industrial bacterial strains. • Research into genes associated with microbial functions. • Generation of data for downstream annotation and phylogenetic studies. • Support for academic, biotechnology, and industrial R&D projects. Sample Requirements • Sample type: Extracted bacterial genomic DNA. • Required DNA quantity: 3 µg per sample. The catalogue does not specify DNA concentration, submission volume, purity thresholds, or sequencing coverage. Confirm these acceptance criteria and the required data output with our team before dispatch. Submission Information Clearly label each sample and provide the isolate identifier, organism name where known, sample source, extraction method, and available DNA quality assessment results. DNA should represent the intended bacterial isolate. Mixed cultures or contamination can complicate genome analysis. Discuss samples of uncertain purity, low DNA quantity, or degraded DNA before submission. Share research objectives and relevant reference genome information. For comparative projects, provide the relationship between isolates and the comparisons required. Sequencing Deliverables • Genomic DNA sequencing library preparation. • 2 × 150 bp paired-end Illumina HiSeq sequencing. Confirm the agreed read count, coverage target, sequence file formats, and data transfer arrangements during project planning, as these are not specified in the catalogue entry. Additional Bioinformatics Analysis The catalogue lists WGS bioinformatics and downstream analysis separately. Depending on the selected package and suitability for the bacterial dataset, analysis may include: • Quality assessment and filtering. • Alignment to a suitable reference genome. • Variant calling, quality assessment, and annotation. • Annotated variant results in spreadsheet format. • Gene annotation and functional analysis. • Phylogenetic analysis. • Screening for virulence-associated genes. • Bacteriocin and CRISPR prediction. • Mobile genetic element identification. • Genome visualisation. If de novo assembly is required, confirm its inclusion and expected outputs before initiating the project. Interpretation Genome recovery and analysis results depend on DNA quality, sequencing depth, contamination, and genome complexity. Short-read sequencing may produce a draft assembly; a complete, closed genome is not guaranteed. Predicted genes indicate genetic potential and require appropriate interpretation alongside experimental evidence. Turnaround Time Sequencing: 3–4 weeks. Bioinformatics analysis: Enquire for the applicable timeline. Service Coverage Based in Bengaluru, we support projects across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai. Contact Us Dextrose Technologies Pvt. Ltd. Phone: +91 9880405558 Email: info.dextrosetech@gmail.com Website: www.dextrosetech.com