Description

Explore bacterial gene expression with Dextrose Technologies Pvt. Ltd. Our prokaryotic transcriptome sequencing service generates RNA sequencing data to support research into microbial responses, expressed genes, and biological differences between experimental conditions. The service combines bacterial ribosomal RNA depletion, RNA-Seq library preparation, and paired-end sequencing. Removing abundant rRNA helps increase the representation of other transcripts in the sequencing dataset. Service Overview Submitted total RNA undergoes bacterial rRNA depletion, followed by RNA-Seq library preparation and 2 × 150 bp paired-end sequencing using the Illumina HiSeq platform specified in our service catalogue. The resulting data provides a foundation for downstream read alignment, transcript abundance estimation, and comparative gene expression analysis. For archaeal samples or other organisms outside the bacterial scope, confirm compatibility of the depletion workflow before submission. Applications • Bacterial gene expression profiling. • Investigation of responses to environmental stress, nutrients, or experimental treatments. • Comparison of microbial cultures under different growth conditions. • Identification of candidate genes associated with a research phenotype. • Studies of industrial strains and fermentation-related responses. • Support for microbiology, biotechnology, dissertation, and PhD research. Sample Requirements • Sample type: Total RNA from the prokaryotic organism under study. • Required quantity: 5 µg total RNA per sample. The catalogue does not specify RNA concentration, submission volume, or an RNA integrity threshold. Confirm acceptance criteria, genomic DNA removal requirements, and transport conditions with our team before dispatch. Submission Information Clearly label each sample and provide the organism name, strain details where available, sample source, extraction method, RNA quantity, and available quality assessment results. For comparative studies, include treatment and control groups, biological replicate information, growth conditions, sampling time points, and research objectives. Share reference genome and annotation details where available. RNA integrity, sample consistency, and experimental design influence the usefulness of the resulting data. Sequencing Deliverables • Bacterial rRNA depletion. • RNA-Seq library preparation. • 2 × 150 bp paired-end sequencing using Illumina HiSeq. • 10 million reads per sample, counted as R1 + R2. The stated output is 10 million individual reads across R1 and R2, rather than 10 million read pairs. The proportion of informative non-rRNA reads depends on sample quality and depletion efficiency. Additional Bioinformatics Analysis The catalogue lists downstream bioinformatics as a separate service. When included in the agreed scope, it covers: • Alignment of quality-filtered reads to a reference genome. • Transcript abundance estimation. • Differential gene expression analysis. • Identification of statistically significant up- and down-regulated genes. • Functional annotation using Gene Ontology and pathway analysis. Statistical comparisons require suitable biological replication and an appropriate study design. Confirm the analysis scope and output formats when planning the project. Turnaround Time Sequencing: 3–4 weeks. Bioinformatics analysis: Enquire for the applicable timeline. Service Coverage Based in Bengaluru, we support projects across India, including Kochi, Kolkata, Delhi, Mumbai, Hyderabad, and Chennai. Contact Us Dextrose Technologies Pvt. Ltd. Phone: +91 9880405558 Email: info.dextrosetech@gmail.com Website: www.dextrosetech.com